Identification and environmental detection of Rhodococcus species by 16S rDNA-targeted PCR
نویسندگان
چکیده
منابع مشابه
Usefulness of strb1 and 16S rDNA-targeted PCR for detection of Streptomyces spp. in environmental samples.
In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification ofa genus-specific sequence in the Streptomyces' 16S rDNA gene. DNA band of the expected size (438 bp) was seen with all the samples. Additionally, specific amplification of the streptomycin-coding...
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DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
متن کاملDetection and identification of Malassezia species in domestic animals and aquatic birds by PCR-RFLP
The present study aimed at detection and species-level identification of the Malassezia yeasts in domestic animals and aquatic birds by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Samples were collected using tape strips and swabs from 471 animals including 97 horses, 102 cattle, 105 sheep, 20 camels, 60 dogs, 30 cats, 1 hamster, 1 squirrel, 50 aquatic birds a...
متن کاملWhat is broad-range 16S rDNA PCR?
Patel A, et al. Arch Dis Child Educ Pract Ed 2017;0:1–4. doi:10.1136/archdischild-2016-312049 INTRODUCTION PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s. Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nuclei...
متن کاملIdentification of Corynebacterium jeikeium from a CAPD patient by 16S rDNA PCR – a case report
Case report A 51-year-old male was admitted in Southern Railway Hospital, Chennai with complaint of abdominal pain for four days. He had a history of renal failure two years before admission and was started on CAPD for end stage renal disease. Prior to admission he was on ambulatory peritoneal dialysis (APD). Peritoneal dialysis (PD) fluid was collected and transported to laboratory aseptically...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 1999
ISSN: 1364-5072
DOI: 10.1046/j.1365-2672.1999.00824.x